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A variety of accessories and solutions to support flow cytometry procedures. Products may include buffer solutions and kits as well as instrument accessories to augment capacity.
The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.
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ViaFluor SE Cell Proliferation Kits use non-toxic, amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. Labeling is covalent, so it withstands fixation and permeabilization for subsequent immunostaining. This kit contains ViaFluor 405 SE which has an excitation/emission of 408/452 nm. The kit contains 10 lyophilized dye vials. Each vial is sufficient for 20 uL of 5000X stock solution.
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Live-or-Dye™ Fixable Viability Staining Kits are cell membrane impermeable amine-reactive dyes designed for discrimination between live and dead cells during flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. Live-or-Dye™ 488/515 is suitable for excitation by 488 nm laser line. This trial size kit has sufficient reagent for 50 assays.
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The Bangs Laboratories Quantum Simply Cellular anti-Human IgG kit is a versatile tool for the quantitative analysis of cellular antigen expression via flow cytometry. This kit provides five calibrated bead populations that mimic human lymphocytes, allowing for accurate Antibody Binding Capacity (ABC) determination and robust standard curves. Its QuickCal analysis template simplifies data interpretation, making it ideal for researchers seeking precise and reliable results in surface marker expression analysis.
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The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain all bacteria. Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. CF594 is spectrally similar to Texas Red, but with brighter fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacteria will be stained with blue fluorescence only. This kit contains sufficient reagent for 800 assays.
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The BD Stem Cell Enumeration Kit is an IVD solution that accurately enumerates viable dual-positive CD45+/CD34+ hematopoietic stem cell populations using flow cytometry. It provides reliable and standardized results, aiding quality assessment in stem cell transplantation processes. The kit minimizes errors through automated calculations and supports analysis of multiple specimen types, differentiating it with its compatibility with various BD flow cytometers and streamlined workflow following ISHAGE guidelines.
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